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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in many tumors, which leads to chromosome instability, and thus might add to tumorigenesis. This protein was observed to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital role in marketing cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two additional splice variants ended up claimed but specific nucleotide sequence data is not really yet readily available.
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Blocking peptides are peptides that bind precisely for the focus on antibody and block antibody binding. These peptide ordinarily is made up of the epitope regarded through the antibody.
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This tutorial outlines further feasible optimization of mobile and immunoassay parameters to make certain the very best results are...
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This manual outlines further more probable optimization of cellular and immunoassay parameters to make certain the absolute best final results are...
Antibodies certain to the blocking peptide now not bind for the epitope on the concentrate on protein. By comparing the staining from your blocked antibody versus the antibody alone, one can see which staining is certain.
Target Information and facts Cyclin E belongs into the really conserved cyclin family, whose members are characterised by a dramatic periodicity in protein abundance with the cell cycle. Cyclins perform as regulators of CDK kinases. Distinct cyclins exhibit distinct expression and degradation patterns which add for the temporal coordination of each and every mitotic occasion. Cyclin E varieties a complex with and features for a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S changeover. This protein accumulates at the G1-S stage boundary and is also degraded as cells development here through S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both of those antibodies...
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